In this exciting role, you will work on solving inspiring scientific challenges to mitigate the level of greenhouse gases using bioengineering. The research project will involve developing synthetic biology tools for microbial metabolic strain engineering of non-model bacteria that consume C1-carbon substrate. You will develop CRISPR based bioengineering methods, biosensors for real time detection of metabolites, and expression of heterologous pathway for biochemical production. You will work in a team with expert scientists, develop state-of-the-art synthetic biology tools and contribute to a global mission of one step forward to reverse climate change.
What You Will Do:
Screen candidate enzymes for heterologous expression in a non-model bacterial host-platform for targeted biochemical production.
Utilize CRISPR and other genetic tools to optimize genetic manipulation protocol for a non-model bacterial host.
Establish use of biosensor for real-time detection of metabolite production.
Maintain an accurate and detailed electronic scientific logbook of all experiments performed. Store all data generated in an organized and secure way. Ensure that others could duplicate results.
Adhere to institutional health and safety requirements.
Ability to conduct practical experiments independently.
Ability to conduct original work that meets the milestones of projects.
Prepare manuscripts for publication and regular progress reports for project.
Additional Responsibilities as needed:
Assist Principal Investigator with training and managing workflow of student scientists.
Participate and present data at group meetings and seminars.
Maintain and support scientific equipment as needed.
What is Required:
PhD in microbiology, biochemistry, molecular and cell biology, synthetic biology and microbial metabolic engineering or a related field received within the last year.
Detailed knowledge of bacterial physiology, genetics, and protein biochemistry.
Experience in genetic tool development of Gram-negative bacteria, including plasmid construction, DNA transformation, genomic integration and deletions, CRISPR and enzyme screening.
Experience with analytical instruments like HPLC, plate readers and spectrophotometers.
Experience with aseptic techniques and cell culturing.
Strong organizational, written and oral communication skills.
Understanding of biotic C1 (CH4) uptake systems.
Experience in gas-fermentation.
Knowledge of bioinformatics tool for plasmid design, DNA sequence analysis and function assessment.
Experience in DNA extraction, metabolite extraction, isolation and structure elucidation (HPLC, LC-MS, GC, and/or GC-MS) and reporter gene assays.
This is a full-time 1year postdoctoral appointment with the possibility of renewal based upon satisfactory job performance, continuing availability of funds and ongoing operational needs. You must have less than 3 years of paid postdoctoral experience. Salary for Postdoctoral positions depends on years of experience post-degree.
This position is represented by a union for collective bargaining purposes.
Salary will be predetermined based on postdoctoral step rates.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Work will be primarily performed at Joint BioEnergy Institute (JBEI) -- Bldg. 978, 5885 Hollis St., 4th floor, Emeryville, CA.
Learn about us!
Biological Systems and Engineering Division
Vision: To lead efforts that combine the power of biology with the tools of engineering to develop sustainable energy and biomanufacturing solutions and improve human health.
Mission:Advance a mechanistic and predictive understanding of complex biological systems over multiple scales in terms of their responses to manipulation, stress, disease and environmental challenges and translate this knowledge using engineering principles to develop resilient systems, tools, and processes for the efficient production of fuels, chemicals, materials, tissues, and therapeutics.
Berkeley Lab is an Equal Opportunity and Affirmative Action Employer. We heartily welcome applications from women, minorities, veterans, and all who would contribute to the Lab's mission of leading scientific discovery, inclusion, and professionalism. In support of our diverse global community, all qualified applicants will be considered for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status.
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.